Growth, sporulation and production of bioactive compounds by Bacillus subtilis R14
نویسندگان
چکیده
منابع مشابه
Single, chemically defined sporulation medium for Bacillus subtilis: growth, sporulation, and extracellular protease production.
The composition and application of a single, chemically defined medium or growth and sporulation of Bacillus subtilis is described. At 37 degrees C cells grew with a doubling time of about 40 min; cultures attained near-maximal spore formation (70 to 80% by 12 h after the end of exponential growth and produced 1 X 10(9) to 2 X 10(9) heat-resistant free spores at 24 h. Dipicolinic acid productio...
متن کاملGrowth and sporulation of entrapped Bacillus subtilis cells.
Growing Bacillus subtilis cells were immobilized in k-carrageenan gel beads. Growth and sporulation of entrapped cells were studied by two different methods: cell enumeration and transmission electron microscopy. Immobilized growing cells had a shorter generation time than free cells did, whereas sporulation timing was unchanged. When steady state was reached, cell density was very high in gel ...
متن کاملBiofilm formation and sporulation in Bacillus subtilis
Biofilms are architecturally complex communities of microorganisms in which the cells are held together by an extracellular matrix, typically containing exopolysaccharides (EPSs), proteins and some nucleic acids. Our understanding of the molecular mechanisms involved in biofilm formation has increased tremendously in recent years. However, information about biofilm formation and sporulation is ...
متن کاملSporulation of Bacillus subtilis in Continuous Culture
Sporulation of Bacillus subtilis 168 was studied in chemostat cultures. Sporulation occurred at high frequency under limitation of growth by glucose or the nitrogen source in minimal medium, whereas rates of sporulation were low for Mg'+, phosphate, citrate, or tryptophan limitation. Sporulation was found at all growth rates tested, and the incidence of spores increased with decrease in growth ...
متن کاملAlcohol-resistant sporulation mutants of Bacillus subtilis.
About 80% of Bacillus subtilis cells form spores when grown in nutrient broth. In medium containing various short-chain aliphatic alcohols, the frequency of sporulation was reduced to 0.5%. Mutants sporulated in the presence of alcohols at a frequency of 30 to 40%. Sporulation in the wild-type cells was sensitive to alcohol at the beginning of sporulation (stage zero). Sensitivity to alcohol in...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Brazilian Archives of Biology and Technology
سال: 2010
ISSN: 1516-8913
DOI: 10.1590/s1516-89132010000300020